Development of eDNA-based forest pest detection tools that use digital technologies (FORSAID)

Invasive pests and pathogens are a growing concern for forest worldwide and in Switzerland. An early detection of such organisms significantly increases chances to prevent and contain the damage they may cause. For their rapid and smooth identification, advanced diagnostic tools are needed that allow the detection of the target species also in complex environments like forests, plantations or urban greens.

A new approach to detect species from environmental samples is via the collection and analysis of so-called environmental DNA (eDNA), which refers to traces of DNA that are left by organisms in their environment. In this project, we aim to harness the potential of eDNA to detect tree pests and pathogens in rainfall and run-off water samples from host trees. More specifically, we will develop and validate molecular-diagnostic assays to identify the emerald ash borer Agrilus planipennis and the chestnut blight fungus Cryphonectria parasitica within the scope of the FORSAID project. Field visits for the collection of eDNA samples to validate and test the developed approaches will also be carried out, in collaboration with the Forest Entomology group at WSL and with international researchers.